Utilization of 5-bromouracil by thymineless bacteria.
نویسندگان
چکیده
Several thymineless Escherichia coli strains have been examined for their ability to replicate their deoxyribonucleic acid when bromouracil is substituted for thymine. The procedure we describe was used to identify a thymineless strain with characteristics relatively favorable to its use in bromouracil labeling experiments. In addition, mutants with an "absolute" thymine requirement could be easily distinguished from one with a "leaky" thymine requirement.
منابع مشابه
Host-induced modification-restriction and the utilization of 5-bromouracil by thymineless mutants of Escherichia coli.
An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K.
متن کاملStudies on ultraviolet irradiation of Escherichia coli containing 5-bromouracil in its DNA.
Thymine-requiring Escherichia coZi 15, tand E. coli B, twhich contained 5-bromouracil in their deoxyribonucleic acid in place of thymine showed a marked increase in sensitivity to ultraviolet (u.v.) radiation. The effect was proportional to the extent of incorporation of 5-bromouracil under defined growth conditions and for a given medium. This increase in sensitivity to U.V. was not hereditary...
متن کامل5-bromouracil utilization by Bacillus subtilis.
The replacement of thymine (T) in deoxyribonucleic acid (DNA) by 5-bromouracil (BU) (F. Weygand, A. Wacker, and H. Dellweg, Z. Naturforsch. 7b:19, 1952) is an aid in density separation of types of DNA, and in studies on radiobiology and mutagenesis. The usefulness of BU is, however, limited by its toxic effects. Extensive substitution in DNA of T by BU is lethal for most Escherichia coli and Ba...
متن کاملThymineless Mutagenesis in Bacteriophage
Thymine deprivation can be achieved in bacteriophage T4 either by the use of the thymidylate synthetase inhibitor FUdR, or by an appropriate combination of genetic blocks; both methods produce marked mutagenesis. Extensive tests of the specificity of thymineless mutagenesis reveal that only A:T base pairs are affected, and that transitions and possibly transversions are produced. This system th...
متن کاملThe recombination of bromouracil-containing deoxyribonucleic acid in Salmonella typhimurium transduction.
Salmonella typhimurium tolerated the replacement of about 70 % of the thymine in the DNA by 5-bromouracil (BU). In phage PLT-22 transduction, the presence of BU in the donor DNA had no effect on the linkage relationship of two contransduced markers and did not conclusively alter the ratio of complete transductants to abortive transductants. The yield of transductants per plaque-forming unit was...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of bacteriology
دوره 93 1 شماره
صفحات -
تاریخ انتشار 1967